South Africa coach Rassie Erasmus trimmed his squad to 33 players on Sunday for the remaining two Tests against England after taking a 1-0 series lead this weekend.
Prop Retshegofaditswe Nche, lock Jason Jenkins and loose forwards Albertus Smith and Nizaam Carr have been released from the original squad chosen for a one-off Wales Test and the England series.
Another prop, Trevor Nyakane, withdrew from the team just before the 42-39 triumph over England in Johannesburg Saturday and has been ruled out of the series with a rib injury.
Veteran hooker Bismarck du Plessis pulled out before the first England international after being injured playing for Montpellier in the French Top 14 final.
Fullback Curwin Bosch, wing Travis Ismaiel and scrumhalf Cameron Wright have also been released while an injury has sidelined France-based centre Francois Steyn last week.
The second Test is at Free State Stadium in Bloemfontein this Saturday and the third at Newlands stadium in Cape Town on June 23.
Forwards: Steven Kitshoff, Siyamthanda Kolisi (captain), Wilco Louw, Frans Malherbe, Mbongeni Mbonambi, Sikhumbuzo Notshe, Pieter-Steph du Toit, Armand van der Merwe, Tendai Mtawarira, Daniel du Preez, Jean-Luc du Preez, Mahlatse Ralepelle, Thomas du Toit, Francois Mostert, Marvin Orie, Teboho Mohoje, Rudolph Snyman, Duane Vermeulen.
Backs: Lukhanyo Am, Andre Esterhuizen, Makazole Mapimpi, Sibusiso Nkosi, Robert du Preez, Warrick Gelant, Jesse Kriel, Embrose Papier, Handre Pollard, Ivan van Zyl, Aphiwe Dyantyi, Elton Jantjies, Damian de Allende, Francois de Klerk, Willie le Roux.